Molekylær karakterisering og diagnosticering af skadegørere

I dette forskningsområde udvikler og anvender vi metoder til fylogenetisk karakterisering, diagnosticering, detektion og kvantificering af planteskadegørere: virus, bakterier, phytoplasma, svampe, nematoder og skadedyr. Vi arbejder hovedsageligt med DNA/RNA-baserede metoder som (real-time)PCR og next-generation-sequencing (NGS). Metoderne understøtter vores myndighedsrådgivning samt erhvervet som helhed til dyrkning af sundere planter. 

Eksempler på afsluttede opgaver:

Gil, J.F., Adams I., Boonham, N., Nielsen S.L., Nicolaisen, M. (2016) Molecular and Biological characterisation of Potato mop-top virus (PMTV, Pomovirus) isolates from the potato growing regions in Colombia. Plant Pathology 65: 1210-1220. 

Sapkota, R., Skantar, A., Nicolaisen, M. (2016) A TaqMan real-time PCR assay for detection of Meloidogyne hapla in root galls and in soil. Nematology 18:147-154

Walter S, Ali S, Kemen E, Nazari K, Bochra B, Enjalbert J, Hansen JG, Brown JKM, Sicheritz-Pontén T, Jones J, de Vallavieille-Pope C, Hovmøller MS, Justesen AF (2016).  Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis, Ecology and Evolution, 6:2790-804

Wieczorek TM,  Jørgensen LN,  Hansen AL, Munk L, Justesen AF (2014). Early detection of sugar beet pathogen Ramularia beticola in leaf and air samples using qPCR", European Journal of Plant Pathology 138:775–785 

Beuch, U., Berlin, S., Åkerblom, J., Nicolaisen, M., Nielsen, S.L., Crosslin, J.M., Hamm, P.B., Santala, J., Valkonen, J.P.T., Kvarnheden, A. (2015) Diversity and evolution of Potato mop-top virus. Archives of Virology 160: 1345-1351

Sapkota, R., Nicolaisen M. (2015) High-throughput sequencing of nematode communities from total soil DNA extractions. BMC Ecology 15:3 

Makarova, O., Contaldo, N., Paltrinieri, S., Kawube, G., Bertaccini, A., Nicolaisen, M (2012) DNA barcoding for identification of ‘Candidatus Phytoplasmas’ using a fragment of the elongation factor Tu gene. PLoS One 12, e52092 

Zijlstra, C., Lund, I., Justesen, A., Nicolaisen, M., Bianciotto, V., Posta, K., Balestrini, R., Przetakiewicz, A., Czembor, E., van de Zande, J., Jensen, P. (2011) Combining novel monitoring tools and precision application technologies for integrated high-tech crop protection in the future (a discussion document). Pest Management Science 67: 616-625 

Nicolaisen, M., Suproniene, S., Nielsen, L.K., Lazzaro, I., Spliid, N.H. and Justesen, A.F. (2009) Real-time PCR for quantification of eleven individual Fusarium species in cereals. Journal of Microbiological Methods 76: 234-240