Protocols
The first early blight subgroup meeting brought together 19 Euroblight members on 19-20 march at Freising, Germany. Over the two days EB field experiments and fungicide ratings were discussed along with increasing problems associated with losses in fungicide sensitivity. In order to improve and standardize the monitoring and diagnostics of EB relevant species lab protocols for testing and comparison are now available on the EuroBlight website. Protocols collated by the Alternaria sub-group is organized according to different topics and they will be further elaborated and new ones will appear whenever appropriate.
Protocol title | Contact person | Download |
Biologicals | ||
Protocol for testing “Effectiveness: leaf late blight” of biologicals (Phytophthora infestans) | Bert Evenhuis, Mout De Vrieze, Hans Hausladen, Ruairidh Bain, Peter Hartvig and Huub Schepers E-Mail: bert.evenhuis@wur.nl | June 2021 |
qPCR | ||
Qualitative PCR diagnostics of A. solani and A. alternata
| Andrea Volz, a.backhaus@wzw.tum.de; Jürgen Leiminger, juergen.leiminger@lfl.bayern.de Lehrstuhl für Phytopathologie, Wissenschaftszentrum Weihenstephan Emil-Ramann-Str. 2 85354-Freising |
June 2014
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Artificial inoculation | ||
Artificial inoculation with Alternaria solani & Alternaria alternata (detached leaf, greenhouse and field)
|
Jan Spoelder, Spoelder@hlbbv.nl
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June 2014
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Artificial inoculation - Greenhouse studies
|
Dr. Birgit Adolf, adolf@wzw.tum.de
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June 2014
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Methods for inoculation of tomatoes and potatoes with Alternaria solani in the greenhouse and field
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Gerd Stammler, Simone Miessner, Franziska Böhme, gerd.stammler@basf.com.
BASF SE, Agricultural Center, Speyerer Strasse 2, 67117 Limburgerhof, Germany
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June 2014
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Protocol for the artificial inoculation with A. solani in field trials (with infected kernels)
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Birgit Adolf, Hans Hausladen
Lehrstuhl für Phytopathologie, Wissenschaftszentrum Weihenstephan, Emil-Ramann-Str. 2. 85354 Freising, Germany. adolf@wzw.tum.de
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July 2015
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Long-Term Storage | ||
Long-term storage of Alternaria isolates |
Andrea Volz, a.backhaus@wzw.tum.de;
|
June 2014
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Long term storage of Alternaria isolates |
Bert Evenhuis
|
June 2014
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Long term storage of Alternaria isolates
| Jan Spoelder, Spoelder@hlbbv.nl HLB Diagnostics Dept. Kampsweg 27 9418 PD Wijster The Netherlands |
June 2014
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Growth and conidia production | ||
Growth and conidia production of Alternaria solani
|
Jan Spoelder,
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June 2014
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Alternaria solani growth and spore production protocol
|
Jürgen Leiminger, juergen.leiminger@lfl.bayern.de
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June2014
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Protocol for conidia production of Alternaria solani
|
Bert Evenhuis
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June 2014
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Protocol for growth and conidia production of Alternaria solani
|
Firuz Odilbekov and Erland Liljeroth, Erland.Liljeroth@slu.se
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June2014
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Isolation | ||
Isolation of A. solani
|
Jürgen Leiminger, juergen.leiminger@lfl.bayern.de
|
June 2014
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Screening for Alternaria solani or alternata
| Bert Evenhuis bert.evenhuis@wur.nl Wageningen UR PPO-AGV-Plant Research International PO BOX 16 6700 AA Wageningen |
June 2014
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Characterization of Cytb mutations | ||
Identification of Alternaria solani GI in combination with analysis of the F129L substitution |
Eva Edin, eva.edin@slu.se,
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June 2014 |
Protocol for the detection of a G143A mutation in Alternaria alternata-isolates
|
Jan Nechwatal,
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June 2014 |
Identification of Alternaria alternata in combination with analysis of the G143A substitution
|
Eva Edin, eva.edin@slu.se,
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June 2014
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Prevalence of the F129L mutation in Alternaria solani from the United States
|
Neil Gudmestad, Neil.Gudmestad@ndsu.edu,
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June 2014
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Confirmation of resistant phenotypes by the proof of cytb mutations (F129L, G143A) in Alternaria isolates
| Dr. Birgit Adolf, adolf@wzw.tum.de Lehrstuhl für Phytopathologie, Wissenschaftszentrum Weihenstephan, Emil-Ramann-Str. 2. 85354 Freising | June 2014 |
